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5–4 μmol/L variety,[156][157] the focus of serotonin in synaptic vesicles was calculated at 270 mM.[182] Following vesicular launch, the resulting focus of serotonin in the synaptic cleft, to which serotonin receptors are uncovered, is estimated to generally be about three hundred μmol/L. Therefore, though in vitro receptor binding affinities
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